Team:Shanghai city/Basic Parts
Team:AtreXtinct/Basic
BBa_K3526000 is a basic part, a special transcription factor AtzR. This part should be used with the part the basic part BBa_K3526003. Under the presence of cyanuric acid (CYA), the AtzR can bind CYA and regulate the promoter PatzD. As a result, the GFP is expressed and emit green fluorescence.
To test the function of part BBa_K3526000 and BBa_K3526003, we build two composite part BBa_K3526004 and BBa_K3526005 into Pseudomonas putida KT2440. After reading related articles, we choose 0μM, 50μM, and 100μM as the detection concentrations of CYA. After preliminary analysis, we obtained Figure 1.
Figure 1
The results of the experiment show that with the presence of part BBa_K3526004 and BBa_K3526005, pseudomonas putida KT2440 can detect cyanuric acid.
Wechat: Search "AtreXtinct" in WeChat